【文献标题】 Chronic restraint stress decreases the repair potential from mesenchymal stem cells on liver injury by inhibiting TGF-b1 generation
【作者 】 X Yang, Z-P Han, S-S Zhang et.al
【影响因子(IF) 】 5.72
【出版期刊】 《Cell Death and Disease》
Materials and Methods
Materials. Dulbecco’s modified eagle medium (low glucose), fetal bovine serum, glutamax (catalog number: 35050-061), penicillin–streptomycin (catalog number:15140-122), trypsin–EDTA (catalog number: 15400-054) and fibroblast growth factor basic (catalog number: PMG0035) were purchased from Gibco (Grand Island, NY, USA). Hydroxyproline detection kit (catalog number: A030-2) was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).Recombinant mouse TGF-b1 (catalog number: 7666-MB-005) was purchased from R&D system (Minneapolis, MN, USA). Mouse TGF-b1 ELISA kit was purchased from Shanghai Hengyuan Biotechnology (Shanghai, China). orticosterone ELISA kit (catalog number: ADI-900-097) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Corticosterone (catalog number: 46148),cortisol (catalog number: C-113), RU486 (catalog number: M8046) were purchased from sigma (St. Louis, MO, USA). Anti-aSMA antibody (catalog number: ab5694) was purchased from Abcam (Cambridge, UK). GFP antibody (catalog number: NB100–1770) was purchased from Novus Biologicals (Littleton,CO, USA). Alexa Fluor 568 goat anti-rabbit IgG(H t L) (catalog: A-11011), Alexa Fluor488 donkey anti-goat IgG(H t L) (catalog number: A-11055) were purchased from Invitrogen (Carlsbad, CA, USA). Smad2/3 antibody (catalog number:5678S),phosphatemad2/3 rabbit mAb (catalog number: 8828S) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-TGF-b1 antibody (catalog number: ab92486) was purchased from Abcam.